Research Interests:

Mechanisms of regulated secretion from endocrine cells. Our primary focus has been on basic cellular and molecular mechanisms underlying vesicle trafficking and recycling pathways and intracellular 2nd messenger signaling in both anterior pituitary cells and pancreatic beta-cells. We are also investigating the cellular mechanisms that control glucagon secretion from pancreatic alpha-cells during hypoglycemia. All projects use a combination of techniques including mammalian cell culture, molecular biology and biochemistry, and quantitative single-cell imaging approaches including immunofluorescence, tracking of GFP-tagged proteins and ratiometric imaging of intracellular ion indicators. We use a variety of imaging technologies including digital-deconvolution combined with wide-field fluorescence and laser scanning confocal microscopy for FRAP and FRET of fluorescent protein-based probes. Projects have been supported by research grants from the National Institutes of Health (NIH), the Juvenile Diabetes Research Foundation International (JDRF) and the Barbara Davis Center for Childhood Diabetes.

Link to Angleson Publication List

Recent Publications:

• Overlease R.L., Bauer R.A., and Angleson J.K. (2005) Retention of peptide hormone during partial secretion in pituitary somatotrophs and corticotrophs. Eur. J. Physiol.(Pfluger’s Arch) 449:458-62 (DOI: 10.1007/s00424-004-1352-8)
• Bauer R.A., Overlease R.L., Lieber J.L., and Angleson J.K. (2004) Retention and stimulus-dependent recycling of dense core vesicle content in neuroendocrine cells. J. of Cell Sci.117, 2193-2202 (DOI: 10.1242/10.1242/jcs.01093)
• Brumback, A.C., Lieber, J.L., Angleson, J.K., and Betz, W.J. (2004) Using FM1-43 to study neuropeptide granule dynamics and exocytosis. Methods 33:287-294. (DOI: 10.1016/j.ymeth.2004.01.002)
• Bauer, R.A., Khera, R.S., Lieber, J.L., and Angleson, J.K. (2004) Recycling of intact dense core vesicles in neurites of NGF-treated PC12 cells FEBS Lett 571:107-111. (DOI: 10.1016/j.febslet.2004.05.086)
• Vo, Y.P., Hutton, J.C., and Angleson, J.K., (2004) Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells. Biochem. Biophy Res. Com.324:1004-1010. (DOI: 10.1016/j.bbrc.2004.09.147)
• Zahn, T.R., Angleson, J.K., MacMorris, MA, Domke1, E., Hutton, JF, Schwartz, C , and Hutton, JC (2004) Dense Core Vesicle Dynamics in Caenorhabditis elegans Neurons and the Role of Kinesin UNC-104. Traffic 5, 544-559. (DOI: 10.1111/j.1600-0854.2004.00195.x)